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A synthetic version of phi29 DNA-packaging motor.  The nano-motor, assembled in vitro using purified or synthetic components, is geared by six ATP-binding RNAs labeled in six different colors.  DNA (cyan/pink complex at the center) is packaged into a protein shell through a 3.6-nm channel.  After conjugation, the motor could have novel applications in nano-technology and gene delivery.
Adapted from:  Hoeprich S and Guo P.2002. Computer Modeling of Three-dimensional Structure of DNA-packaging RNA(pRNA) Monomer, Dimer, and Hexamer of Phi29 DNA Packaging Motor. The Journal of Biological Chemistry. 277(23):20794-20803.




The potential use of pRNA hexamers as polyvalent gene delivery vectors.  Six copies of pRNA have been found to form a hexameric ring to drive the DNA-packaging motor of bacterial virus phi29.  There would therefore be six positions available to carry foreign moieties for targeting, therapy, and detection. 
Guo, S., Tschammer, N., Mohammed, S. & Guo, P. 2005. Specific delivery of therapeutic RNAs to cells via the dimerization mechanism of phi29 motor pRNA. Human Gene Therapy. 16:1097- 1109.


An array composed of five connectors of bacteriophage phi29 DNA packaging motor.  Each connector contains twelve copies of the protein subunit gp10, shown with the complete sequence of 309 amino acids.  One connector at the center is viewed from the narrow end of the connector, while four connectors at the corners of the square are viewed from the wide end. 
Guo Y, Blocker F, Xiao F and Guo P. 2005. Construction and 3-D computer modeling of connector arrays with tetragonal to decagonal transition induced by pRNA of phi29 DNA-packaging motor. Journal of Nanoscience and Nanotechnology. Vol.5, 856-863.

 

 

 

RNA as building blocks of nanostructures of different sizes and shapes
(MSNBC) (NSF News)

 

 

 

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