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A synthetic version of phi29 DNA-packaging
motor. The nano-motor, assembled in vitro using purified or
synthetic components, is geared by six ATP-binding RNAs labeled in
six different colors. DNA (cyan/pink complex at the center) is
packaged into a protein shell through a 3.6-nm channel. After
conjugation, the motor could have novel applications in
nano-technology and gene delivery.
Adapted from: Hoeprich
S and Guo P.2002. Computer Modeling of Three-dimensional Structure
of DNA-packaging RNA(pRNA) Monomer, Dimer, and Hexamer of Phi29 DNA
Packaging Motor. The Journal of Biological Chemistry.
277(23):20794-20803.
The potential use of pRNA hexamers as
polyvalent gene delivery vectors. Six copies of pRNA have been
found to form a hexameric ring to drive the DNA-packaging motor of
bacterial virus phi29. There would therefore be six positions
available to carry foreign moieties for targeting, therapy, and
detection.
Guo,
S., Tschammer, N., Mohammed, S. & Guo, P. 2005. Specific
delivery of therapeutic RNAs to cells via the dimerization mechanism
of phi29 motor pRNA. Human Gene Therapy. 16:1097-
1109.
An array composed of five connectors of
bacteriophage phi29 DNA packaging motor. Each connector
contains twelve copies of the protein subunit gp10, shown with the
complete sequence of 309 amino acids. One connector at the
center is viewed from the narrow end of the connector, while four
connectors at the corners of the square are viewed from the wide
end.
Guo
Y, Blocker F, Xiao F and Guo P. 2005. Construction and 3-D computer
modeling of connector arrays with tetragonal to decagonal transition
induced by pRNA of phi29 DNA-packaging motor. Journal of
Nanoscience and Nanotechnology. Vol.5, 856-863.
RNA
as building blocks of nanostructures of different sizes and
shapes
(MSNBC) (NSF News)
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